What should we do when receiving tissue culture plants?


  • Step 1. Do not do any thing 2-3 days to keep them adjust with new temp. Keep them in light not direct sun (you can use a dedicated lights)
  • Step 2. After that start to open and close for more 2-3 days. (lids flask/plastic jip)
  • Step 3. Transfer then grow them
  • Notice: Keep it like that when you receive it, do not open it. Keep it some light. not direct sun Let’s plants adjust with new environtment


My box/flask have broken when I receive, what should be done?

The first thing, you should check the plants healthy or not, broken jelly, mold or not and follow our instructions.

Better if possible transfer the plants to outside according to our next tutorial bellow

My box/flask have broken jelly when I receive, what should be done?

In the event of a broken jelly, you can keep shorter step 1 only one day then start Step 2

That mean, keep them adjust 1 days with new temperature, then open/close the box for 2 days.
(some of my customer even jelly broke that still keep then for a week – in the case TC plants are healthy and without problem with mold)

My box/flask have mold when I receive, what should be done?

If have mold, this case can not wait, take out and wash it then plant it right away, dip in captan and trichoderma harzianum before you transfer them, see bellow

My tissue culture plants don’t have the variegated shown?

All tissue culture is exactly of mother plant have variegated
Almost TC plants have the variegation from 80-90% , some of plants have the variegation is not stable (we noted in product listing), but it is in 10-20% of variegated when growing to 4-6 inches pot, you won’t easily see them now, but it won’t let you down. Yellow and green plants may be transformed into variegated and varigated maybe re appear
All Thai people are selling as same as our tissue culture plants, their rate are low but it’s possible.
Stay calm for this job.

What is the ratios of the potting mix and substances can use to transfers into?

Option 1,
In 10 portions, we mix 5 portions for coconut husk chips,be sure its in small pieces, 3 portions of perlite, 1 pertion of sand, and 1 pertion of peat moss or coco- peat
If you can’t find coconut husk chips, substitute with equal portion of perlite and peat moss
Then add a bit of slow release fertilizer

Option 2,
1. Planting material, in 10 parts
peat moss = 7 parts
perlite = 2 parts
sand = 1 part
2. 100 ml of water , 7-10 drops of betadine
3. B1 (root accelerator) mixed according to the proportion on the side
Tips to look at humidity of planting material
– The moisture of planting material must rise but not wet
– Check by gently holding the planting material in your hand so that the water does not flow when the material is realeased
– When the hands are realeased, the planting material will remain stable and will not split apart.

Option 3,
Transfer to sphagnum moss only (for experience people)

Before proceeding:
Take the tissue culture out of the container, wash the stock thoroughly, try to remove all the causes of mold
Dip in captan and Trichoderma harzianum

What is detail of Step by Step can I do ?

PREPARING – Your equipment
– 3 or 4 cups of water
– Scissors to cut bag
– Betadine or anti fungus (captan and trichoderma harzianum)
– Rooting hormone
– Sphagnum moss, peat moss or coco peat (soak into water – squeeze out and rinse a few times)
– Tweezers if necessary
– Chopsticks
– A small paint brush if necessary
STEP 1– Open the package – wash out jelly food thoroughly. If you have a mini paint brush it is helpful.
Rinse until they’re clean.
STEP 2– Soak them in anti fungus for 15 mins (Betadine mix with water will also work)
STEP 3– Soak them in rooting hormone
STEP 4– Plant them into media of choice. (see 3 options)
STEP 5– Put them into closed package (plastic container or plastic bag) – allowing light pass through. Bright indirect light or under a grow light.

FINALLY – AFTER 3 weeks if you have noticed new shoots of atleast 2 or 3 new leaves then they are ready to leave their container- slowly open them up – allowing them to adjust to their new environment. Keep them around 50-60 percent humidity if possible.
Spray or give rooting hormone from time to time (every 2 weeks )

Important – must clean out the jelly as much as possible or you may experience fungus.
DO NOT over soak with water – your plants need humidity but leaving them too wet for too long may cause rot.

What other ways can I do?

👌The processing of saplings nursery for tissue culture plants:
1. Finely grated coconut flakes or sphagnum moss (peat moss is not recommended because a lot of water 💦will get wet and can easily become moldy)
2. A clear plastic cup with a tight lid. Or planting trays using plastic bags to cover baking.
3. Antifungal :captan or any brand of Mancozep.
4. B1 root accelerator
5. Paintbrush
6. Tissue paper
🕵️Checking tissue culture plants before transfer to media:
The sapling comes in a sterile bag. Contains food agar and root accelerator can be planted and left to grow in bags for a long time until the food agar runs out or becomes infected.
Do not cut open the bag, put it in a bright place out of direct sunlight, good ventilation, not stuffy. until the roots are ready for planting and upright
If the sapling has been traumatized by transport, the bag is torn, the plant or leaf is fallen, bruised, has mold or a jelly that changes color, becomes cloudy, pink, red, and black, it can be planted in media immediately.
Media (planting meterial) method:
Step 1. Mix the antifungal drug in 1 liter of water according to the ratio, soak the coconut husks for 1-2 hours, then squeeze until the water does not drip.
Step 2. In the evening, cut off the end of the bag. Or slightly open the bottle to let the air in and the plants adjust. In the morning, remove the seedlings from the bottle/bag. Wash all food agar. Use a brush to rake in the crevices. Do it gently, being careful not to break roots or leaves. Yellow, bruised, torn leaves, discarded, then rinsed with clean water 1-2 more times.
Step 3. Mix together antifungal and root accelerator B1 according to the ratio in a bowl. Soak the washed seedlings for 5 minutes.
Step 4. Prepare a saucer with tissue paper, place the sprouts, let them damp. Use a tissue to absorb the water so that there are no stagnant water at the roots and leaves.
Step 5. Prepare a container for planting, bring a glass or a seedling tray into the coconut husks without pressing. Make a hole by using the tip of the brush to dig into the coconut husks.
Step 6. Plant the sapling, take the root into the hole. Use the tip of the brush to gently brush the coconut husks over the roots.
Step 7. Spray with a spray of fungicide and the remaining B1 root accelerator, as long as it is moist, do not let it get wet. Do not apply any fertilizer during the nursery period.
Step 8. Close the lid or put a plastic bag over the mouth of the baking bag. placed in a well-lit place out of direct sunlight Good ventilation, not stuffy, not hot
Step 9. Keep an eye out for symptoms.
9.1 If there is a yellow card with a withered card dead plant to be discarded If dry, no condensation, spray a little more water.
9.2 If there is mold, wash the seedlings thoroughly, soak them for 5 minutes, and then plant them in new planting material.
Step 10. 7 days, the shoots and leaves will look upright, raised up, the top leaves slightly larger, 2-3 new leaves are nursed and survived.
Step 11. Wait until there are 3-4 new leaves or about 3 weeks before planting and changing pots. Change the planting material, add peas moss, perlite, stones, pine bark, clay tablets as you like. and start fertilizing You can continue to close the lid and slowly open the lid at night for 2-3 nights, close it during the day. therefore can be taken out of the closed container


Propagation the Tissue Culture plants

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