What other ways can I do?
👌The processing of saplings nursery for tissue culture plants:
1. Finely grated coconut flakes or sphagnum moss (peat moss is not recommended because a lot of water 💦will get wet and can easily become moldy)
2. A clear plastic cup with a tight lid. Or planting trays using plastic bags to cover baking.
3. Antifungal: captan or any brand of Mancozep.
4. B1 root accelerator
6. Tissue paper
🕵️Checking tissue culture plants before transfer to media:
The sapling comes in a sterile bag. Contains food agar and root accelerator can be planted and left to grow in bags for a long time until the food agar runs out or becomes infected.
Do not cut open the bag, put it in a bright place out of direct sunlight, good ventilation, not stuffy. until the roots are ready for planting and upright
If the sapling has been traumatized by transport, the bag is torn, the plant or leaf is fallen, bruised, has mold or jelly that changes color, becomes cloudy, pink, red, and black, it can be planted in media immediately.
Media (planting meterial) method:
Step 1. Mix the antifungal drug in 1 liter of water according to the ratio, soak the coconut husks for 1-2 hours, then squeeze until the water does not drip.
Step 2. In the evening, cut off the end of the bag. Or slightly open the bottle to let the air in and the plants adjust. In the morning, remove the seedlings from the bottle/bag. Wash all food agar. Use a brush to rake in the crevices. Do it gently, being careful not to break roots or leaves. Yellow, bruised, torn leaves, discarded, then rinsed with clean water 1-2 more times.
Step 3. Mix together antifungal and root accelerator B1 according to the ratio in a bowl. Soak the washed seedlings for 5 minutes.
Step 4. Prepare a saucer with tissue paper, place the sprouts, let them damp. Use a tissue to absorb the water so that there are no stagnant water at the roots and leaves.
Step 5. Prepare a container for planting, bring a glass or a seedling tray into the coconut husks without pressing. Make a hole by using the tip of the brush to dig into the coconut husks.
Step 6. Plant the sapling, take the root into the hole. Use the tip of the brush to gently brush the coconut husks over the roots.
Step 7. Spray with a spray of fungicide and the remaining B1 root accelerator, as long as it is moist, do not let it get wet. Do not apply any fertilizer during the nursery period.
Step 8. Close the lid or put a plastic bag over the mouth of the baking bag. placed in a well-lit place out of direct sunlight good ventilation, not stuffy, not hot
Step 9. Keep an eye out for symptoms.
9.1 If there is a yellow card with a withered card dead plant to be discarded If dry, no condensation, spray a little more water.
9.2 If there is mold, wash the seedlings thoroughly, soak them for 5 minutes, and then plant them in new planting material.
Step 10. 7 days, the shoots and leaves will look upright, raised up, the top leaves slightly larger, 2-3 new leaves are nursed and survived.
Step 11. Wait until there are 3-4 new leaves or about 3 weeks before planting and changing pots includes change the planting material, add peas moss, perlite, stones, pine bark, clay tablets as you like. and start fertilizing. You can continue to close the lid and slowly open the lid at night for 2-3 nights, close it during the day therefore can be taken out of the closed container