WHAT SHOULD WE DO WHEN RECEIVING TISSUE CULTURE PLANTS?

Instructions:

  • STEP 1. Do not do anything 1-2 days to keep them adjusted with the new temp. Keep them in light, not the direct sun (you can use a dedicated light)
  • STEP 2. Open the package – wash out jelly food thoroughly. If you have a mini paintbrush it is helpful.
    Rinse until they’re clean.
  • STEP 3. Soak them in anti-fungus for 15 mins (Betadine mix with water will also work)
  • STEP 4. Soak them in rooting hormone
  • STEP 5. Plant them into media of choice. (see 3 options)
  • STEP 6. Put them into the closed package (plastic container or plastic bag) – allowing light to pass through. Bright indirect light or under a grow light.
    LEARN MORE BELOW

FREQUENTLY ASKED QUESTIONS

My box/flask has broken when I receive it, what should be done?

step1, you should check whether the plants are healthy or not, broken jelly, mold or not, and follow our instructions.

Better if possible transfer the plants outside to media according to our next tutorial below

My box/flask has broken jelly when I receive it, what should be done?

In the event of a broken jelly, you can keep shorter step 1 for only one day then start step 2

mean, keep them adjust for 1 day with the new temperature, then open/close the box
(some of my customers even jelly broke that still keep then for a week – in the case TC plants are healthy and without problem with mold)

My boxes/flasks have mold when I receive them, what should be done?

If it has mold, please can not wait, take out and wash it then go to step 2 right away, dip it in anti-fungus before you transfer them, see instructions

Don’t have the variegated shown on tissue culture plants?

All tissue culture is exactly of mother plant have variegated
Almost TC plants have the variegation from 80-90%, some of the plants have the variegation is not stable (we noted in the product listing), but it is in 10-20% of variegated when growing to 4-6 inches pot, you won’t easily see them now, but it won’t let you down. The yellow and green plants’ leaves may be transformed into variegated and variegation maybe reappear
All Thai people are selling as same as our tissue culture plants, their rate is low but it’s possible.
Stay calm for this job.

What are the ratios of the potting mix and substances that can use to transfer into?

Option 1,

In 10 portions, we mix 5 portions for coconut husk chips, be sure it’s in small pieces, 3 portions of perlite, 1 portion of sand, and 1 portion of peat moss or coco-peat
If you can’t find coconut husk chips, substitute with an equal portion of perlite and peat moss [5:5]
Then add a bit of slow-release fertilizer

Option 2,
1. Planting material, in 10 parts
peat moss = 7 parts
perlite = 2 parts
sand = 1 part
2. 100 ml of water, 7-10 drops of betadine
3. B1 (root accelerator) mixed according to the proportion on the side
Tips to look at the humidity of planting material
– The moisture of planting material must rise but not be wet
– Checking by gently holding the planting material in your hand so that the water does not flow when the material is released
– When the hands are released, the planting material will remain stable and will not split apart.

Option 3,
Transfer to sphagnum moss only (for experienced people)

Notice:
Before proceeding:
Take the tissue culture out of the container, wash the stock thoroughly, try to remove all the causes of mold
Dip in anti-fungus

What is the detail of step by step can I do?

PREPARING – Your equipment
– 3 or 4 cups of water
– Scissors to cut the bag
– Betadine or anti-fungus (captan and trichoderma harzianum)
– Rooting hormone
– Sphagnum moss, peat moss, or coco peat (soak into the water – squeeze out and rinse a few times)
– Tweezers if necessary
– Chopsticks
– A small paintbrush if necessary
STEP 1– Open the package – and wash out jelly food thoroughly. If you have a mini paintbrush it is helpful.
Rinse until they’re clean.
STEP 2- Soak them in anti-fungus for 15 mins (Betadine mix with water will also work)
STEP 3- Soak them in rooting hormone
STEP 4- Plant them into the media of choice. (see 3 options)
STEP 5– Put them into the closed package (plastic container or plastic bag) – allowing light to pass through. Bright indirect light or under a grow light.

FINALLY – AFTER 3 weeks if you have noticed new shoots of at least 2 or 3 new leaves then they are ready to leave their container- slowly open them up – allowing them to adjust to their new environment. Keep them around 50-60 percent humidity if possible.
Spray or give rooting hormone from time to time (every 2 weeks )

Important – must clean out the jelly as much as possible or you may experience fungus.
DO NOT oversoak with water – your plants need humidity but leaving them too wet for too long may cause rot.

What other ways can I do?

👌The processing of acclimated for tissue culture plants:
Equipment:
1. Finely grated coconut flakes or sphagnum moss (peat moss is not recommended because a lot of water 💦will get wet and can easily become moldy)
2. A clear plastic cup with a tight lid. Or planting trays using plastic bags to cover baking.
3. Antifungal: captan or any brand of Mancozeb.
4. B1 root accelerator
5. Paintbrush
6. Tissue paper
🕵️Checking tissue culture plants before acclimated:
The sapling comes in a sterile bag. Contains food agar and root accelerator can be planted and left to grow in bags for a long time until the food agar runs out or becomes infected.
Do not cut open the bag, put it in a bright place out of direct sunlight, with good ventilation, not stuffy. until the roots are ready for planting and upright
If the sapling has been traumatized by transport, the bag is torn, the plant or leaf is fallen, bruised, has mold or jelly that changes color, becomes cloudy, pink, red, or black, it can be planted in media immediately.
Growing Media (Potting soil) method:
Step 1. Mix the antifungal drug in 1 liter of water according to the ratio, soak the coconut husks for 1-2 hours, then squeeze until the water does not drip.
Step 2. In the evening, cut off the end of the bag. Or slightly open the bottle to let the air in and the plants adjust. In the morning, remove the seedlings from the bottle/bag. Wash all food agar. Use a brush to rake in the crevices. Do it gently, being careful not to break roots or leaves. Yellow, bruised, torn leaves, discarded, then rinsed with clean water 1-2 more times.
Step 3. Mix together antifungal and root accelerator B1 according to the ratio in a bowl. Soak the washed seedlings for 5 minutes.
Step 4. Prepare a saucer with tissue paper, place the sprouts, and let them damp. Use a tissue to absorb the water so that there is no stagnant water at the roots and leaves.
Step 5. Prepare a container for planting, bring a glass or a seedling tray into the coconut husks without pressing. Make a hole by using the tip of the brush to dig into the coconut husks.
Step 6. Plant the sapling, and take the root into the hole. Use the tip of the brush to gently brush the coconut husks over the roots.
Step 7. Spray with a spray of fungicide and the remaining B1 root accelerator, as long as it is moist, do not let it get wet. Do not apply any fertilizer during the nursery period.
Step 8. Close the lid or put a plastic bag over the mouth of the baking bag. placed in a well-lit place out of direct sunlight good ventilation, not stuffy, not hot
Step 9. Keep an eye out for symptoms.
9.1 If there is a yellow card with a withered card dead plant is to be discarded If dry, no condensation, spray a little more water.
9.2 If there is mold, wash the seedlings thoroughly, soak them for 5 minutes, and then plant them in new planting material.
Step 10. In 7 days, the shoots and leaves will look upright, raised up, the top leaves slightly larger, and 2-3 new leaves are nursed and survived.
Step 11. Wait until there are 3-4 new leaves or about 3 weeks before planting and changing pots includes changing the planting material, adding peas moss, perlite, stones, pine bark, and clay tablets as you like. and start fertilizing. You can continue to close the lid and slowly open the lid at night for 2-3 nights, close it during the day therefore can be taken out of the closed container

OPTION 2: ACCLIMATED METHOD DETAILS WITH PICTURES:

Propagation the Tissue Culture plants

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